Extended Data Fig. 9: Hypocretin, bone marrow neutrophils and HCRTR1.
From: Sleep modulates haematopoiesis and protects against atherosclerosis
a, Flow cytometry gating strategy for bone marrow pre-neutrophils, immature neutrophils and mature neutrophils. b, HCRTR1 (GFP) in bone marrow and blood neutrophils from WT;bmHcrtr1GFP/GFP mice. c, mRNA expression in cultured bone marrow pre-neutrophils exposed to LPS and/or HCRT-1 (for untreated n = 3 mice, except Mpo, n = 6 mice; for HCRT-1, n = 3 mice, expect Csf1, n = 4 mice and Mpo, n = 6 mice; for LPS, n = 3 mice except Csf1, n = 7 mice and Mpo, n = 6 mice; for LPS and HCRT-1, n = 3 mice, except Csf1, n = 11 mice, Csf2, n = 4 mice and Mpo, n = 6 mice). d, Csf1 expression in sorted bone marrow cells of wild-type and Hcrt−/− mice (n = 5 wild-type mice; n = 6 Hcrt−/− mice). e, Analysis of mRNA transcript expression in bone marrow leukocytes of Apoe−/− mice after 16 weeks of sleep fragmentation (for Apoe−/−, n = 5 mice, except Il10, Il34, Cxcl12, Csf3, n = 4 mice and Csf1, n = 9 mice; for Apoe−/− SF mice, n = 6 mice, except Il5, Il1β, Il6, Il34, Cxcl12, Csf3, n = 5, Il10, n = 5 mice and Csf1, n = 12). nd, not detected. f, Blood neutrophils in WT;bmHcrtr1GFP/GFP mice over 24 h (n = 3 per group). Data presented as mean ± s.e.m., **P < 0.01, one-way ANOVA.
