Extended Data Fig. 4: Sleep fragmentation does not alter bone structure and does not depend on the microbiome.
From: Sleep modulates haematopoiesis and protects against atherosclerosis
a, b, μCT analysis of trabeculae (a) and cortical bone structure (b) of Apoe−/− mice and Apoe−/− mice after 16 weeks of sleep fragmentation. The bone volume fraction (BV/TV), bone mineral density (BMD), trabecular number (Tb.N), trabecular thickness (Tb.Th), structural model index (SMI), cortical tissue mineral density (Ct.TMD), cortical area (Ct.Ar), total area (T.Ar), cortical thickness (Ct.Th) and cortical porosity (Ct.Porosity) were analysed (n = 9 per group). c, d, Analysis of leukocytosis in SF (c) and Hcrt−/− (d) mice at ZT3 after receiving a cocktail of antibiotics in drinking water for 4 weeks (n = 3 per group). Data are mean ± s.e.m., *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed Mann–Whitney U-tests.
