Extended Data Fig. 2: Order of the DncV, cGAS and CdnE reactions.
From: Bacterial cGAS-like enzymes synthesize diverse nucleotide signals
a–c, Incubation of CD-NTase enzymes with non-hydrolysable nucleotides traps reaction intermediates and identifies the order of the reaction. Left, PEI–cellulose TLC analysis of reactions as in Fig. 1b, in which individual NTPs have been replaced by their non-hydrolysable nucleotide analogues. Exposed γ-phosphates are labelled in parentheses to indicate that these are removed by phosphatase treatment before analysis. Data are representative of three independent experiments. Right, previously determined reaction mechanisms (DncV and cGAS)10,51 and proposed reaction mechanism for CdnE.
