Extended Data Fig. 1: Further characterization of tau pathologies in CTE cases 1–3.
From: Novel tau filament fold in chronic traumatic encephalopathy encloses hydrophobic molecules
Coexisting pathologies. a, Staining of tau inclusions in the temporal cortex of CTE cases 1, 2 and 3 using antibodies RD3 (three-repeat tau), anti-4R (four-repeat tau) and AT8 (pS202 and pT205 tau). Scale bar, 50 μm. b, Gallyas–Braak silver staining of tau inclusions in the temporal cortex of CTE cases 2 and 3. Scale bar, 50 μm. c, Staining of tau inclusions in the spinal cord of CTE cases 1 and 2 using antibody AT8. Scale bar, 50 μm. d, Immunoblots of sarkosyl-insoluble tau from the temporal cortices of CTE cases 1, 2 and 3 using antibodies BR133, BR134 and AT8. e, Staining of a TDP-43 inclusion in the spinal cord of CTE case 1. Scale bar, 25 μm. f, Staining of CTE case 2 for poly-GA inclusions in the cerebellum and TDP-43 inclusions in the spinal cord, temporal cortex, hippocampus and amygdala. Top right, double-labelling of tau inclusions (AT8, brown) and TDP-43 inclusions (red) is shown. Scale bars, 25 μm (top), 50 μm (bottom). g, Staining of CTE case 3 for α-synuclein inclusions in the substantia nigra, dorsal motor nucleus (DMN) of the vagus nerve and locus coeruleus. Scale bar, 50 μm. Nuclei were counterstained blue in all images.
