Extended Data Fig. 7: In vivo photocrosslinking experiments show that LPS passes through the β-jellyroll domains of LptF and LptC, but not LptG.

a, Blots from Fig. 3c are shown for reference. b, In vivo photocrosslinking experiments performed analogously to those in Fig. 3b, c. The UV-photocrosslinkable amino acid pBPA was substituted at positions in the periplasmic β-jellyroll domains of LptC, LptF and LptG. Positions in the interior of the LptF and LptC can crosslink to LPS, whereas those facing outside the β-jellyroll do not. No crosslinks to LPS were observed at ten positions in the LptG β-jellyroll; by contrast, LptG(S30pBPA), in TM1 and facing the cavity of the transmembrane domain, can crosslink LPS. c, Ribbon diagram of the structure of the V. cholerae complex, showing the β-jellyroll domains of LptC, LptF, and LptG. Side chains of residues that crosslink LPS are shown in red, whereas residues that do not crosslink LPS are shown in blue. Blots shown in a and b are representative of data from three biological replicates.