Extended Data Fig. 1: Imaging results and theoretical modelling for several Tribolium wild-type specimens.
From: Attachment of the blastoderm to the vitelline envelope affects gastrulation of insects
a–c, Kymographs of myosin intensity (coloured according to the colour bar at the bottom) along the contour of the blastoderm for three different Tribolium wild-type specimens injected with mRNA that encodes for Tcsqh-eGFP, recorded with light-sheet microscopy at 25 °C. White lines show flow, measured by particle image velocimetry tracking. The horizontal dashed lines denote the individual time points displayed in d–f. Panels a–c represent three independent experiments, n = 3. d–f, Experimentally determined myosin intensity (colour) as well as tissue flow field (arrows) for single time points of a–c. Insets, experimentally determined flow field compared to theoretical prediction, assuming the tissue is anchored at the anterior–ventral side of the egg (red anchor). Fitting parameters were vc = 0.35 μm s−1, α < 0.2 (d); vc = 0.88 μm s−1, α < 0.2 (e); and vc = 0.45 μm s−1, α < 0.2 (f). Data from a and d are shown in Fig. 1c, e, g and Supplementary Video 3. Data from b are shown in Supplementary Video 1. Note that our theory can recapitulate the flow fields of individual embryos, as opposed to using ensemble-averaged data.
