Extended Data Fig. 2: Imaging results and theoretical modelling for several Tribolium specimens with Tcif knockdown.
From: Attachment of the blastoderm to the vitelline envelope affects gastrulation of insects
a–c, Kymographs of myosin intensity (coloured according to the colour bar at the bottom) along the contour of the blastoderm for three different Tribolium specimens injected with a mixture of mRNA that encodes for Tcsqh-eGFP and iBeetle RNAi against Tcif, recorded with light-sheet microscopy. The temperature of experiments was 30 °C (a) or 25 °C (b, c). White lines show flow, measured by particle image velocimetry tracking. The horizontal dashed lines denote the individual time points displayed in d–f. Panels a–c represent three independent experiments, n = 3. d–f, Experimentally determined myosin intensity (colour) as well as tissue flow field (arrows) for single time points of a–c. Insets, experimentally determined flow field compared to theoretical prediction assuming the tissue is free to flow with respect to the vitelline envelope. Fitting parameters were vc = 0.45 μm s−1, α < 0.2 (d); vc = 0.58 μm s−1, α < 0.2 (e); vc = 0.39 μm s−1, α < 0.2 (f). Data from d are shown in Fig. 3g and Supplementary Video 12.
