Extended Data Fig. 6: Functional effects of CD19 acquisition by CAR T cells.
From: CAR T cell trogocytosis and cooperative killing regulate tumour antigen escape
a, Diagram illustrating transduction of T cells with retroviral vector encoding CD19 under a PGK-100 promoter. Representative flow cytometry profile of CD19 expression on transduced T cells (n = 6 donors). b, Percentage of PD-1, LAG-3 and TIM-3 expression in trog+ and trog− fractions (n = 4 donors). c, Diagram illustrating co-expression of CD19 and CAR on T cells. T cells were transduced with SFG vector expressing CD19 followed by CAR transduction 24 h later. After transduction, cells were left in culture for 6 days. d, Absolute count of CAR T cells (n = 6 donors). e, Percentage of T cells expressing of PD-1, LAG-3 and TIM-3 (n = 6 donors). P values were determined by two-sided Mann–Whitney U-test. Data are mean ± s.e.m.
