Extended Data Fig. 7: Internalized CD19–CAR complex after trogocytosis.
From: CAR T cell trogocytosis and cooperative killing regulate tumour antigen escape
a, Left, MFI of mCherry gated on CAR T cells after co-culture with NALM6 cells expressing CD19–mCherry. Right, MFI of goat anti-mouse IgG (GAM) gated on CAR T cells (n = 3 independent samples, data are representative of three donors). b, Left, representative FACS profile of CAR T cells stained with GAM and LNGFR in the presence or absence of NALM6 cells (data are representative of n = 4 mice per group). Right, MFI of GAM on CAR T cells isolated from mice bone marrow or extramedullary tumour sites by the time of relapse (n = 4 mice per group). c, MFI of GAM on CAR T cells co-cultured with autologous blasts derived from refractory/relapsed patients with ALL and CLL (n = 4 patient samples). d, Representative confocal microscopy images of NALM6 cell–CAR T cell conjugates. Data are representative of three donors (ten cells per experiments). e, Diagram illustrating CD19 trogocytosis by CAR T cells. P values were determined by two-sided Mann–Whitney U-test. Data are mean ± s.e.m.
