Extended Data Fig. 6: Mutant mRNA decay helps confer genetic robustness.

a, qPCR analysis of Fermt2 and Fermt1 mRNA expression levels following CRISPR interference-mediated knockdown of Fermt2 transcription in Fermt2 knockout cells. b, qPCR analysis of emilin3a, emilin3b and emilin2a mRNA expression levels in 20 hpf wild types, egfl7^Δ4 mutants and egfl7^{full locus del.} mutants. c, Number of central arteries (CtAs) connecting to the basilar artery (BA) in 58 hpf vegfaa^Δ10 and vegfaa^{promoter-less} mutants. d, Blood-flow velocity in 78 hpf wild types, hbegfa^Δ7 mutants and hbegfa^{full locus del.} mutants. e, Quantification of the cardiac ventricle length in 100 hpf wild types, alcama ^Δ8 mutants and alcama^{promoter-less} mutants. Wild-type or control expression levels were set at 1 for each assay (a, b). n = 3 (a, b); n = 13 (vegfaa^Δ10−/−) and 19 (vegfaa^{promoter-less−/−}) (c); n = 25 (d); and n = 18 (wild-type siblings of alcama^Δ8−/−), 7 (alcama^Δ8−/−), 22 (wild-type siblings of alcama^{promoter-less−/−}) and 15 (alcama^{promoter-less−/−}) (e) animals. Data are mean ± s.d., and a two-tailed Student’s t-test was used to calculate P values.

Source data