Extended Data Fig. 3: Transcriptional adaptation involves enhanced transcription and is independent of the DNA lesion itself.
From: Genetic compensation triggered by mutant mRNA degradation
a, qPCR analysis of hbegfb and emilin3a mRNA and pre-mRNA expression levels in hbegfa and egfl7 wild-type and mutant zebrafish. b, qPCR analysis of Fermt1 and Rel mRNA and pre-mRNA expression levels in Fermt2 and Rela wild-type and knockout cells. c, IGV tracks of the Fermt1 locus showing ATAC-seq signals in wild-type and Fermt2 knockout cells. d, qPCR analysis of hbegfa, hbegfb, egfl7 and emilin3a mRNA expression levels in hbegfa and egfl7 wild-type and Δ3 mutant zebrafish. e, qPCR analysis of vegfaa, vegfab, egfl7 and emilin3a mRNA expression levels in vegfaa and egfl7 wild-type and 5′UTR mutant zebrafish. f, qPCR analysis of vcla and vclb mRNA expression levels in vcla wild-type and last exon (exon 22) mutant zebrafish. n = 3 biologically independent samples. Wild-type expression levels were set at 1 for each assay. Data are mean ± s.d., and a two-tailed Student’s t-test was used to calculate P values.
