Extended Data Fig. 4: Reduction in mutant transcript levels is caused by mRNA decay.
From: Genetic compensation triggered by mutant mRNA degradation
a, qPCR analysis of hbegfa, egfl7 and alcama mRNA and pre-mRNA expression levels in hbegfa, egfl7 and alcama wild-type and mutant zebrafish. b, qPCR analysis of Fermt2 and Rela mRNA and pre-mRNA expression levels in Fermt2 and Rela wild-type and knockout cells. c, qPCR analysis of 4sU-labelled Fermt2, Rela and Actg1 mRNA and pre-mRNA expression levels in Fermt2, Rela and Actg1 wild-type and knockout cells. d, Fitted exponential decay curves of Fermt2 mRNA expression levels in wild-type and Fermt2 knockout cells. t1/2, half-life. e, Fitted exponential decay curves of Rela mRNA expression levels in wild-type and Rela knockout cells. f, Fitted exponential decay curves of Actg1 mRNA expression levels in wild-type and Actg1 knockout cells. n = 3 (a, b, d–f) or n = 2 (c) biologically independent samples. Wild-type expression levels were set at 1 for each assay (a–c). Data are mean ± s.d., and a two-tailed Student’s t-test was used to calculate P values.
