Extended Data Fig. 7: Microvascular reperfusion and cytoarchitectonics in the occipital neocortex and cerebellar cortex.

a, Micro-CTA demonstrates patency of major arterial vessels and arterioles in the occipital lobe and cerebellum. OL, occipital lobe; V, vermis; LC, lateral cortex; CP, choroid plexus; L, left; R, right. b, c, Intravascular haemoglobin fluorescent signal in the occipital neocortex (b) and cerebellar cortex (c), demonstrating signal in precapillary arterioles (arrowheads) and capillaries (arrows) in both 10 h PMI controls and BEx brains. Images are from a representative brain from each condition; experiments were repeated in n = 3 independent brains per group. d, Nissl stains of the occipital neocortex with higher magnification of boxed area below, showing preserved regional cytoarchitectonics and neuronal structure in BEx brains. Pyramidal-shaped cell bodies as well as apical and basal dendrites are present in both 1 h PMI and BEx brains. An inverted pyramidal neuron structure is also appreciable under BEx perfusion (arrow). Scale bars, 350 μm (top), 100 μm (bottom). Images are from a representative brain from each condition; experiments were repeated in n = 3 independent brains per group. e, Representative Nissl stains of the cerebellar cortex reveal preserved Purkinje cell structure (arrows) in 1 h PMI and BEx brains, when compared to 10 h PMI and CP conditions. Images are from a representative brain from each condition; experiments were repeated in n = 3 independent brains per group.