Extended Data Fig. 3: WRN depletion preferentially impairs MSI cell viability.
From: WRN helicase is a synthetic lethal target in microsatellite unstable cancers
a, Immunoblot of WRN and GAPDH levels 4 days after sgRNA transduction. b, Relative viability following sgRNA transduction in a competitive growth assay. Data are mean ± s.e.m. (n = 6 biological replicates). Comparison between WRN sgRNAs and negative controls at day 10; two-way ANOVA; *P = 0.37, †P = 1.2 × 10−7, ‡P = 0.23, §P = 2.7 × 10−19. c, Clonogenic assay after shRNA transduction with a non-targeting negative control (RFP shRNA (shRFP)), a pan-essential control (PSMD2 shRNA (shPSMD2)) and two shRNAs against WRN (shWRN1 and shWRN2). d, Relative staining intensity of the clonogenic assay. Data are mean ± s.e.m. (n = 3 technical replicates). Representative data from one experiment are shown. All experiments were performed three times.
