Extended Data Fig. 5: Characterization of COL2.3+ subpopulations and cycling cells.
From: The bone marrow microenvironment at single-cell resolution
a, Relative average scRNA-seq expression levels of O1- (Edil3, Mmp14, Ostn, Col12a1, Angptl2 and Col16a1), O2- (Sox9, Comp, Chad and Col10a1) and O3- (Col11a2, Col1a2, Sparc, Bglap2 and Bglap3) associated genes. b–d, Average scRNA-seq expression levels (n = 9,622 cells) and bone marrow immunofluorescence of MMP14 (green) (b) (n = 3 mice), CD9 (green) (c) (n = 3 mice) and CAR3 (green) (d) (n = 3 mice) in COL2.3–tdTomato femur, with arrows indicating co-staining with tdTomato (red). Nuclei, DAPI (blue). Arrowhead, COL2.3+MMP14+ (b), COL2.3+CD9+ (c) and COL2.3+CAR3+ (d). e, Expression levels of Mki67 in all identified subpopulations. n = 9,622 cells. f, Enriched Gene Ontology biological processes terms that are most-strongly associated with cycling cluster (C), colour-coded by the significance of enrichment and size on the basis of the fraction of overlapping genes. n = 9,622 cells. g, Contribution of VE-Cad–tdTomato+, LEPR–tdTomato+ and COL2.3–tdTomato+ cells to the cycling cluster at a steady state (n = 70 cells). The data in b–e are mean ± s.e.m.
