Extended Data Fig. 6: Effect of treatment with 5-FU on subsets of the bone marrow niche.

a, Representative haematoxylin and eosin-stained sections of bone marrow on day five after treatment with control (PBS) or 5-FU (n = 3 mice). b, Frequency and numbers of bone marrow LSK cells on day five after treatment with control (CNTRL) (n = 4) or 5-FU (n = 5). c, Absolute numbers of bone marrow niche cells, vascular VE-Cad⁺ (control, n = 4; 5-FU, n = 10), perivascular LEPR⁺ (control, n = 2; 5-FU, n = 5) and COL2.3⁺ osteoblasts (control, n = 4; 5-FU, n = 5) from mice treated with PBS or 5-FU. d, Gene signatures of LEPR⁺ subpopulations (including cluster P5) on the basis of the average relative expression levels of the ten most-significant markers for each cluster, exclusively within the LEPR⁺ subset. MAST with Bonferroni correction. e, Relative expression levels of upregulated adipogenesis-associated genes and downregulated osteogenesis-associated genes in LEPR⁺ subpopulations in response to treatment with 5-FU. f, Pathways enriched in LEPR⁺ cells in response to treatment with 5-FU (n = 17,374 cells). Fisher’s exact test. g, Contribution of VE-Cad–tdTomato⁺, LEPR–tdTomato⁺ and COL2.3–tdTomato⁺ cells to the cycling cluster after treatment with 5-FU (n = 418 cells). h, Expression levels of Mki67 in all identified subpopulations at a steady state, and after treatment with 5-FU (n = 17,374 cells). The data are mean ± s.d. N.S., not significant, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. Student’s t-test, two-tailed (b, c). The data in h are mean ± s.e.m.

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