Extended Data Fig. 7: Validation of scRNA-seq in VE-Cad+ and LEPR+ cells following treatment.
From: The bone marrow microenvironment at single-cell resolution
a, b, Heat map and hierarchical clustering of mean normalized expression values of VE-Cad+ (a) and LEPR+ (b) control-treated and 5-FU-treated samples for 5-FU-modulated genes (top 50 differentially expressed) in two independent scRNA-seq experiments. c, d, log-transformed fold changes of differentially expressed genes (up- and downregulated > 1.2×, adjusted P value < 0.001) in 5-FU-treated versus control-treated VE-Cad+ (n = 697 genes) cells (c) and LEPR+ (n = 829 genes) cells (d), for two independent experiments. The trend line (dashed) and the confidence interval (grey shading) were calculated using the linear model. MAST with Bonferroni correction. VE-Cad+ control-treated, n = 5,796 cells; VE-Cad+ 5-FU-treated, n = 1,481 cells; LEPR+ control-treated, n = 6,128 cells; LEPR+ 5-FU-treated, n = 4,867 cells.
