Extended Data Fig. 3: Characterization of VE-Cad⁺ subpopulations.

a, Relative average scRNA-seq expression levels of the previously described arterial and sinusoidal gene signatures at a steady state, within the VE-Cad⁺ clusters V1 and V2. b, Average scRNA-seq expression levels (left) (n = 4,669 cells) and bone marrow immunofluorescence (right) of arterial expression of SCA-1 (Ly6a), CD102 (Icam2) and PODXL (Podxl) (n = 3 mice); LAMA1 staining (blue) labels all bone vessels; yellow arrowheads indicate arterial vessels. Dashed lines mark bone marrow (bm), compact bone (cb), growth plate (gp) and metaphyseal (mp) bone regions. c, Bone marrow immunofluorescence of arterioles co-stained with SCA-1 and PODXL. n = 3 mice. d, Average scRNA-seq expression levels (left) and bone marrow immunofluorescence (right) of sinusoidal VEGFR3 (Flt4) (red) and CD54 (Icam1) (green) markers. n = 3 mice. LAMA1 staining (blue) labels all bone vessels. e, Average scRNA-seq expression levels and representative flow cytometry analysis of the arterial subpopulation (V1) using SCA-1 and scRNA-seq-identified LY6C (Ly6c1) and CD34 (Cd34) from VE-Cad–tdTomato bone marrow (n = 3 mice). Cells were pre-gated on DAPI⁻tdTomato^high cells. The data in b, d, e are mean ± s.e.m.