Extended Data Fig. 8: Effect of selective gLN removal on lymph flow, SFB colonization and oral tolerance.
From: Compartmentalized gut lymph node drainage dictates adaptive immune responses
a, Fast Green tracing of ileal lymphatic drainage to the gLNs of SPF C57BL/6 mice, 5 min after injection of 3 µl Fast Green in sham-operated (left, biological duplicates; Extended Data Fig. 2g) or ΔicLN (middle and right, biological triplicates) mice 3 weeks after surgery. Red arrows, sites of injection. b, c, Relative quantification of SFB-specific 16S in luminal contents of indicated gut segments from recently SFB-colonized SPF C57BL/6 Jax mice (b, n = 13 per group from four independent experiments) or parentally colonized Taconic mice (c, n = 7 per group from four independent experiments) after sham or ΔicLN surgery. d–i, Total eosinophils (d) and dendritic cells (e) in BALF and frequency of eosinophils (f) and dendritic cells (g) among CD45+ cells in lungs, and total IgE (h) and anti-OVA IgG1 (i) in serum from SPF C57BL/6 mice subjected to oral tolerance as in Fig. 4k but without S. venezuelensis infection, 14 days after sham surgery (n = 7), I- and C-gLN removal (ΔicLN, n = 5) or D-gLN removal (ΔdLN, n = 6). Data are representative of two independent experiments. ns, not significant (ANOVA).
