Extended Data Fig. 9: Extended analysis of gLN swelling and restructuring, and dendritic cell and CD4⁺ T cell subset frequencies, upon S. venezuelensis infection.

a, Indicated gLN positions in non-infected (N.I.) SPF C57BL/6 mice or mice infected with 700 S. venezuelensis (S.v.) larvae 8 days earlier. b, 3D reconstruction of vasculature (anti-CD31) after solvent clearing (iDISCO+) and light sheet microscopy of indicated gLNs from non-infected SPF C57BL/6 mice or SPF C57BL/6 mice infected with 700 S. venezuelensis larvae 14 days earlier; scale bars, 500 μm. c, Quantification of mature S. venezuelensis worms in gut epithelium 8 days after infection with 700 larvae. n = 8, pooled from two independent experiments. d–g, Frequency of CD11c⁺ cells among CD45⁺ cells (d), and CD11c⁺ (e) and CD8α⁺(f) cells among MHCII^intCD11c⁺ cells, or GATA3⁺FOXP3⁺ cells among TCRβ⁺CD4⁺ cells (g) in non-infected SPF C57BL/6 mice or mice infected with 700 S. venezuelensis larvae 8 days earlier; n = 5. Data are representative of three independent experiments. h–l, Frequency of CD103⁺CD11b⁺ (h), CD103⁺CD11b^– (i), or CD103^–CD11b⁺ cells (j) among MHCII^intCD11c⁺ dendritic cells, and of GATA3⁺FOXP3^– (k) or GATA3⁺FOXP3⁺ (l) cells among TCRβ⁺CD4⁺ cells in denoted gLNs of SPF C57BL/6 mice infected with 700 S. venezuelensis larvae 21–50 days earlier, as indicated (n = 4 per time point) or non-infected (day 21) SPF C57BL/6 mice. *P < 0.05, **P < 0.01, ***P < 0.005; ns, not significant (one-tailed t-test or ANOVA).