Extended Data Fig. 7: LIF blockade profoundly affects gene expression in cancer cells analysed by RNA-seq.
From: Targeting LIF-mediated paracrine interaction for pancreatic cancer therapy and monitoring
a–e, Differential gene expression comparing the EpCAM+ cancer cells purified from individual tumours of KPf/fCL mice treated with either chemo (GI) or combo (GA) is shown in heat maps. Colours correspond to standardized expression of genes. a, Hierarchical clustering of all 1,624 differentially expressed genes (false discovery rate, FDR <0.05, log2(fold change) >0.8 and FPKM >2 in at least four samples). b, GSEA. Heat maps presenting the expression levels of genes related to tumour-initiating cell markers (c), STAT3 downstream targets (d) and Gem response (e). f, Venn diagram comparing the differentially expressed genes by pharmacologic LIF blockade and genetic Lifr deficiency revealed 811 consensus genes. g, Hierarchical clustering of 811 consensus genes with differential expression by pharmacologic LIF blockade and genetic Lifr deficiency.
