Extended Data Fig. 2: Atherosclerosis is promoted by endothelial SR-B1 but not by endothelial PDZK1, and neither endothelial SR-B1 nor PDZK1 affects circulating lipids.
From: SR-B1 drives endothelial cell LDL transcytosis via DOCK4 to promote atherosclerosis
Findings are shown for SR-B1 in mixed background females (a–g) and C57BL/6 males (h–n), and for PDZK1 in C57BL6 males (o–u). a, h, o, Representative in situ aortic arch images of atherosclerotic plaque (yellow arrows); b, i, p, representative images for lipid-stained lesions in en face aortas; d, k, r, representative images for lipid and haematoxylin-stained aortic root sections (lesions outlined by yellow dashed line, magnification 40×). c, j, q, Quantification of lesion areas in en face aortas (per cent total surface area); c, n = 13 and 14 for SR-B1fl/fl and SR-B1∆EC, respectively; j, n = 10 and 9, respectively; q, n = 14 per group. e, l, s, Quantification of lesion areas in in aortic root sections; e, n = 9 per group; l, n = 10 and 9 for SR-B1fl/fl and SR-B1∆EC, respectively; s, n = 10 and 11, respectively. f, m, t, Plasma total cholesterol (TC), triglyceride, and HDL cholesterol (HDL-c). f, n = 12 per group for TC and TG, 10 per group for HDL-c; m, n = 16 and 9 for SR-B1fl/fl and SR-B1∆EC, respectively, for TC and TG, and n = 10 and 9, respectively, for HDL-c; t, n = 14 per group for TC and TG, and 10 per group for HDL-c. Findings for SR-B1fl/fl, SR-B1ΔEC and PDZK1ΔEC are shown in blue, red and orange, respectively. g, n, u, Representative lipoprotein profiles. Data are mean ± s.e.m; in c, e, j, l, P values calculated by two-sided Student’s t-test.
