Extended Data Fig. 2: Calibration lines for carboxylic acids.
From: Synthesis and breakdown of universal metabolic precursors promoted by iron
Shown are the correlations between the concentrations of aqueous solutions of carboxylic acids (glyoxylic, glycolic, oxalic, malonic, levulinic, mesaconic, and hydroxyketoglutaric plus oxopentenedioic acids) and the measured GC peak area. Error bars correspond to the standard deviation (three independent runs). Orange lines show the 95% confidence bounds computed for second-degree polynomial fits (OriginPro). Calibration lines for glycine, aspartic acid and glutamic acid are shown in Supplementary Fig. 10. Calibration lines for the remaining compounds detected here (pyruvate, malate, fumarate, succinate, α-ketoglutarate, isocitrate, cis-aconitate, tricarballylate and alanine) are identical to those we reported previously for the same analytical set-up9 (Supplementary Table 1).
