Extended Data Fig. 1: Clstn3b encodes an adipocyte-specific protein.
From: Innervation of thermogenic adipose tissue via a calsyntenin 3β–S100b axis
a, Quantitative PCR analysis of Clstn3b expression in wild-type and Lsd1-knockout BAT (n = 3 mice). b, Histone marker and transcription regulator ChIP–seq at the Clstn3 locus from BAT. c, Quantitative PCR analysis of Clstn3b expression in inguinal subcutaneous WAT from mice acclimatized to room temperature or 4 °C (n = 4 mice). d, Mass spectrometry identification of CLSTN3β peptides. e, Conservation of CLSTN3β within the mammalian class. The red cross and green ticks indicates the absence and presence, respectively, of homologues of CLSTN3β in mammalian subclasses. f, Sequence alignment between the unique exon of Clstn3b from human, and a fragment, in an intron upstream of the penultimate exon of Clstn1, in the genome of Chinese softshell turtle. Note how the position of this fragment corresponds to the β-selective exon in Clstn3. All data are mean ± s.e.m. Statistical significance was calculated by unpaired Student’s two-sided t-test.
