Extended Data Fig. 7: tdTomato-expressing neural precursors migrate from the SVZ towards olfactory bulbs.
From: Progenitors from the central nervous system drive neurogenesis in cancer
a, Immunostaining of neural precursors, transduced by a tdTomato-expressing lentiviral vector administered by stereotaxic injection into the SVZ of tamoxifen-injected DCX-creERT2;loxp-EYFP Hi-MYC cancer mice, tdTomato+ cells leave the SVZ to reach the olfactory bulb area through the physiological rostral migratory stream, and differentiate into neurons in olfactory bulbs (top, white arrows, tdTomato+eYFP+ cells). Note that these tdTomato-expressing neural cells do not spread in the dentate gyrus (bottom). Scale bars, 20 μm. b, c, FACS plots showing tamoxifen-induced eYFP+ (green), lentiviral vector-transduced tdTomato+ (red, tdTomato+) and eYFP+tdTomato+ (yellow) neural precursors in the SVZ, olfactory bulbs and dentate gyrus of five-month-old Hi-MYC mice (b) and infiltration of these cells into prostate tumours (c). d, Lineage tracing of Lin−tdTomato+ cells that infiltrate prostate tumours but not healthy tissues surrounding the tumour (testis, epididymis and cremaster). Data are mean + s.e.m. Student’s t-test (one-sided, no adjustment). Sample sizes are listed in Source Data. ***P < 0.001. e, Summary of the five developmental stages of neural progenitors migrating (through the blood) from the central nervous system to the tumour during cancer development. tdTom+, tdTomato+.
