Extended Data Fig. 8: Lin−tdTomato+ cells cannot reach the tumour after lentiviral injection in the dentate gyrus.
From: Progenitors from the central nervous system drive neurogenesis in cancer
a, Frequencies of Lin−tdTomato+ cells in prostate tumours at 16 weeks of age, after tdTomato+ lentiviral vector injection by stereotaxy into the dentate gyrus (n = 6) at week 5 after birth, by comparison to an injection into the SVZ (n = 10 mice). Data are mean + s.e.m. Student’s t-test (one-sided, no adjustment). ***P < 0.001. b, Absolute numbers of Lin−tdTomato+ cells in the SVZ (n = 9) or dentate gyrus (n = 8) 16 weeks after the injection of the tdTomato+ lentiviral vector in the respective brain area. Data are mean + s.e.m. c. Respective mean fluorescence intensities (MFI) for tdTomato signal in SVZ (MFI/SVZ, red) and dentate gyrus (MFI/DG, grey) shown in b. d, Dentate gyrus vascularization (CD31+, pink) in one-year-old Hi-MYC mice does not exhibit any leakiness of albumin (orange) in the dentate gyrus. DCX–eYFP+ cells (green). DAPI, dark blue. Scale bar, 30 μm. e, The blood–brain barrier is breached in the SVZ of Hi-MYC cancer mice. Combined injection of fluorescein–albumin (65 kDa, green) and TRITC–dextran (4.4 kDa, red) reveals extravasation of both dyes in SVZ tissues (top) throughout sinusoids. In the dentate gyrus, the dyes do not spread outside of the capillaries (bottom). DAPI, dark blue. Scale bars, 20 μm. tdTom+, tdTomato+.
