Extended Data Fig. 9: Lin− tdTomato+ cells can infiltrate Pten prostate tumours, PyMT breast tumours and metastases.
From: Progenitors from the central nervous system drive neurogenesis in cancer
a, b, After injection of a tdTomato-expressing lentiviral vector by stereotaxy into the SVZ at week 5 after birth, Lin−tdTomato+ cells are found at week 16 after birth in Pten prostate tumour tissues (a) or PyMT breast tumour tissues (b), by contrast to wild-type prostate or mammary pad tissues. c, Frequencies of Lin−tdTomato+ cells in Pten mice (n = 5) or wild-type littermates (n = 8). Data are mean + s.e.m. Student’s t-test (one-sided, no adjustment). **P < 0.01. Lin−tdTomato+ cells are also found in metastases. d, e, Luciferase-expressing human PC3 cancer cells orthotopically xenografted in the mouse prostate disseminate to colon, liver, lung and lymph nodes between 12 to 14 weeks after graft (d) and metastatic tissues were carefully dissected before be stained with a combination of haematoxylin and eosin (e). Scale bar, 300 μm. f, Neural progenitors transduced by a tdTomato-expressing lentiviral vector administered by stereotaxy into the SVZ, travel to PC3 xenografts and developing metastases in colon, liver, lung and lymph nodes at weeks 12–14 after graft. g, Frequencies of Lin−tdTomato+ cells in tumour tissues, listed above, by comparison to healthy tissues. Data are mean + s.e.m. Student’s t-test (one-sided, no adjustment). *P < 0.05, **P < 0.01. Sample sizes per group are listed in Source Data. tdTom+, tdTomato+.
