Extended Data Fig. 1: DCX+ cells express INA, a neural precursor marker, but not NF-H, a neuron-specific marker, in human prostate and Hi-MYC mouse prostate tumours, and are associated with tumour aggressiveness.
From: Progenitors from the central nervous system drive neurogenesis in cancer
a, In human adenocarcinoma, DCX+ cells (green) and large peri-tumour nerve fibres (white arrow) express INA (red), a marker of developing neural cells and mature fibres. By contrast, mature nerve fibres express the neuron-specific cytoskeletal subunit of NF-H (light blue) but not DCX, a specific marker of neural progenitors in the central nervous system. DAPI, dark blue. Scale bar, 20 μm. b, Early time point analysis of recurrence-free survival of patients with high-risk prostate cancer. Early recurrence after radical prostatectomy is associated with high density of DCX+ cells (>20 DCX+ cells per field, n = 13). P = 0.0338, log-rank (Mantel–Cox). This is the same cohort of patients as shown in Fig. 1i, but here is focused on high-risk tumours. c, d, DCX+ cells are also present in mouse prostate tumours and are stained for PSA-NCAM (red) (c) and INA (red) (d) but do not express mature nerve markers such as NF-H (light blue), which suggests that they are neural progenitors. DCX, green; DAPI, dark blue. Scale bar, 20 μm. Three independent experiments.
