Extended Data Fig. 2: Characterization and quantification of DCX–eYFP+-expressing cells in a Cre-lox mouse model of cancer.
From: Progenitors from the central nervous system drive neurogenesis in cancer
a, Triple-transgenic DCX-creERT2;loxp-eYFP Hi-MYC mouse model of cancer mice is generated by crossing mice expressing MYC under the probasin promoter (AAR2/PBSN–MYC) with mice that express tamoxifen-inducible CreERT2 recombinase under the control of the DCX promoter/enhancer and bred with mice that have the eYFP gene inserted in the Gt(Rosa)26Sor locus with an upstream loxP-flanked STOP sequence (top). Timeline of tamoxifen injections (bottom). b. Schematics of axial, coronal and sagittal sections of the adult mouse brain showing the olfactory bulbs and olfactory tract (OT), the SVZ along the lateral ventricle, and the dentate gyrus. c. Images of DCX–eYFP+ neural precursors (yellow), in olfactory bulbs, from mice described in a, co-stained with anti-PSA-NCAM (green) and anti-INA (red) antibodies. DAPI, dark blue. Scale bar, 20 μm. Three independent experiments. d, FACS plots of eYFP+ cells isolated from Lin− cells from the brain (SVZ, olfactory bulbs, dentate gyrus and tumour tissues of DCX-creERT2;loxp-eYFP Hi-MYC mice (red) compared to control littermates (green and yellow). e, Quantification of Lin−eYFP+ cells in SVZ, olfactory bulbs and dentate gyrus (top) and prostate tumour (bottom). Data are mean + s.e.m. Student’s t-test (one-sided, no adjustment). Sample sizes are listed in Source Data. f, Lin−eYFP+ cells in Hi-MYC prostate tumours do not derive from tumour cells. Real-time quantitative PCR analyses of mRNA extracted from Lin−eYFP+ cells isolated from prostate tumour tissues, olfactory bulbs and the SVZ of DCX-creERT2;loxp-eYFP Hi-MYC mice. The Lin−eYFP+ cells do not express MYC oncogene or the cytokeratins (CK5 and CK18) present in the Lin+eYFP− fraction that comprises prostate epithelial cells. Data are mean + s.e.m.
