Extended Data Fig. 7: Analysis of the accumulation of the Mdj1 precursor.

a, Left, Mdj1 precursor in post-nuclear supernatant and mitochondrial fraction. The data are representative of two independent experiments. Right, degradation of the Mdj1 precursors by proteinase K (prot. K) in pam17∆ ubx2∆ cell extracts. The data are representative of three independent experiments. b, Mdj1 precursor levels in cell extracts from wild-type, pam17∆, pam17∆ ubx2∆ and pam17∆ ubx2∆ + UBX2 strains expressing Ubx2. The data are representative of three independent experiments. c–e, Degradation kinetics of the Mdj1 precursor were analysed in cell extracts from the indicated mutant strains. The data are representative of three (c, d) or two (e) independent experiments.