Extended Data Fig. 1: Partner proteins and stability of Ubx2.
From: Mitochondrial protein translocation-associated degradation
a, c, Affinity purifications of lysed wild-type, HA–Tom5, HA–Tom6, HA–Tom7 (a) and Por1–HA (c) mitochondria. The data are representative of three independent experiments. Load 4%; elution 100%. b, Co-immunoprecipitation with Tom22 antiserum of lysed wild-type mitochondria. The data are representative of two independent experiments. Load 2%; elution 100%. d, Wild-type and ubx2∆ cell extracts and mitochondria were analysed by SDS–PAGE, and immunodetection with Ubx2-specific antiserum. The data are representative of two independent experiments. Asterisk marks an unspecific band recognized by the Ubx2 antiserum. e, Ubx2 stability in intact cells or ruptured cells incubated for the indicated time points. The data are representative of three independent experiments. f, Affinity purifications of lysed wild-type, Hrd1–HA, Ubx2–HA and Doa10ProtA cell extracts. The data are representative of three (Hrd1–HA) or two (Doa10ProtA) independent experiments. Load 1%; elution 100%.
