Extended Data Fig. 1: SHMT2 exists in a dimer–tetramer equilibrium.

a, Structure of SHMT2ΔN(A285T) dimer (PDB code 6DK3). Unbiased electron density maps are coloured green. The structure was erroneously deposited by the Structural Genomics Consortium (SGC) as wild type with PDB code 3OU5. It was corrected and redeposited with PDB code 6DK3 on 28 May 2018. b, Structure of wild-type SHMT2α tetramer (PDB code 4PVF) bound to PLP. Regions in red show structural rearrangements upon PLP binding. c, Native mass spectrometry spectra (single measurement experiment) of different forms of SHMT2ΔN (top) and measured and calculated masses (bottom). Calculated masses nearest to the measured masses are underlined. d, Size-exclusion chromatography profiles of different forms of SHMT2ΔN (top) and a Coomassie-stained SDS–PAGE gel (bottom). Data are representative of three independent experiments. e, Comparison of SHMT2 protomers with and without PLP.