Extended Data Fig. 4: Electron microscopy analysis of BRISC and BRISC–SHMT2 complex. | Nature

Extended Data Fig. 4: Electron microscopy analysis of BRISC and BRISC–SHMT2 complex.

From: Metabolic control of BRISC–SHMT2 assembly regulates immune signalling

Extended Data Fig. 4

a, Negative-stain electron microscopy analysis. Selected 2D-averaged classes of BRISCΔNΔC (left) and BRISCΔNΔC–SHMT2ΔN(A285T) (right). b, Representative cryo-EM micrograph. Green circles (180 Å diameter) indicate auto-picked particles. c, Flow chart of data processing. Final electron microscopy maps, coloured according to local resolution, are provided (scale bar in Å). d, FSC curves for the indicated maps. The final resolution was calculated using the gold-standard FSC cut-off at 0.143 frequency.

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