Extended Data Fig. 6: Comparison of the ester hydrolysis reaction catalysed by OE1.3 and OE1.3(C186A/C212A).
From: Design and evolution of an enzyme with a non-canonical organocatalytic mechanism
Time course of the hydrolysis of fluorescein 2-phenylacetate (100 µM) catalysed by OE1.3 (purple) and OE1.3(C186A/C212A) (black) (0.1 µM) in PBS pH 7.4, 22 °C, showing that the Cys186Ala and Cys212Ala mutations have a negligible effect on catalytic activity.
