Extended Data Fig. 3: Kinetic characterization of OE1, OE1 variants and CUT190.
From: Design and evolution of an enzyme with a non-canonical organocatalytic mechanism
a–f, Michaelis–Menten plots of fluorescein 2-phenylacetate hydrolysis catalysed by OE1 (a), OE1.1 (b), OE1.2 (c), OE1.3 (d), OE1.3(N46E) (e) and OE1.3(Y45F) (f). g, A Hill plot of fluorescein 2-phenylacetate hydrolysis catalysed by CUT190, n = 2.3 ± 0.4. Data are mean ± s.d. of measurements made in triplicate. h, A table summarizing the kinetic parameters of OE1 and its variants, and the cutinase variant Ser226Pro/Arg228Ser (CUT190).
