Extended Data Table 1 Experimental conditions used for substrate profiling of OE1 and OE1.3

From: Design and evolution of an enzyme with a non-canonical organocatalytic mechanism

  1. Assay parameters used to determine the initial activity (ν0/[E]) for OE1- and OE1.3-catalysed reactions with a range of fluorescein esters.
  2. aAr, fluorescein.
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