Extended Data Fig. 2: Effects of FAPα cell deletion.
From: Distinct fibroblast subsets drive inflammation and damage in arthritis
a–c, Change in wrist and ankle joint thickness during STIA with AUC analysis following treatment with diphtheria toxin (FAPα cell deletion) at days 5 and 7 (a), and days 10 and 12 (b), or prophylactically before the induction of STIA (c) (all, n = 8, mice per group). d, Representative time-course analysis of structural joint damage assessed by micro-CT following treatment with diphtheria toxin (FAPα cell deletion) at days 3 and 5 following induction of STIA. e, Quantification of bone erosion and new bone formation (e; n = 8 mice per group), combined for front and hind paws. f, Histological examination of ankle joint tissue sections at day 12 STIA with quantification of bone erosion, pannus formation and bone formation (all by H&E) and cartilage destruction (by safranin O staining) (n = 12 mice per group). g, Representative images of cathepsin K immunohistochemical staining of osteoclasts (brown) in the ankle joints of day 12 STIA mice. h, Number of osteoclasts (cathepsin K positive) per tissue section in DTR− versus DTR+ mice at day 12 STIA compared to non-arthritic control mice (n = 12 mice per group). i, Expression of bone turnover markers including osteoclast and osteoblast markers in whole paw tissue analysed by RT−qPCR (n = 8 mice per group, data are expressed as mean fold change in expression compared to expression in non-arthritic mice). Statistics: Mann–Whitney test (a–c); two-way ANOVA with Tukey’s post hoc test (e); one-way ANOVA with Tukey’s post hoc test (f, h). Data are mean ± s.d., except AUC analysis in a–c, which are shown as box plots (centre line, median; box limits, upper and lower quartiles; whiskers, maximum and minimum values).
