Extended Data Fig. 2: Summary of founder and germline-transmitted SHANK3 mutations.
From: Atypical behaviour and connectivity in SHANK3-mutant macaques
a, Schematic showing the structure of the wild-type macaque SHANK3 gene and magnified panels with the annotated sequence of the gRNA and protospacer adjacent motif (PAM) for both strands within exon 21. b, SURVEYOR assay showing SpCas9-mediated indels in cultured cynomolgus monkey primary skin fibroblasts with indicated gRNAs. c, Genotyping PCR results of individual monkey embryos injected with a mixture of SpCas9 mRNA, SHANK3 gRNA no. 1 and gRNA no. 2. Asterisks indicate effectively edited embryos. d, Number of injected embryos, transferred recipients and newborn macaques in this study. e, SHANK3-mutant macaques have similar body weights to those of age-matched wild-type controls. f, Pie charts of genotype (indels) of DNA from semen from mutant macaques M2 and M3 show a similar pattern to their respective blood samples.
