Extended Data Fig. 6: Expression of transfected vectors and mutation types of off-target RNA SNVs in single cells.
From: Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis
a, Expression of GFP, APOBEC1 and TadA–TadA* was quantified in all sequenced single cells. Thresholds are indicated by blue dashed lines. Thresholds of log2 (FPKM + 1) for GFP, BE3 and ABE7.10 are 0.3, 1 and 0.3, respectively. Cells with expression levels higher than the threshold were included for further analysis. b, c, Cells with high expression of TadA–TadA* or APOBEC1 showed greater numbers of RNA SNVs than those with low expressions in the ABE7.10 (n = 9 cells) or BE3 group (n = 4 cells), respectively. Box-and-whisker plots: centre line indicates median value, box represents first and third quantile, whisker indicates maximum and minimum values. d, Distribution of mutation types for GFP-transfected single cells (n = 16 cells). e, Distribution of mutation types for BE3–site 3-transfected single cells (n = 31 cells). Cells with expression of APOBEC1 higher than the threshold are included in the red square. f, Distribution of mutation types for ABE7.10–site 1-transfected single cells (n = 28 cells). Cells with expression of TadA–TadA* higher than the threshold are included in the red square. The number indicates the percentage of a certain type of mutation among all mutations. SC, single cell.
