Extended Data Fig. 10: DNA on-target and RNA off-target activities of different engineered variants.
From: Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis
a, Comparison of the width of editing windows between ABE7.10 and ABE7.10F148A. n = 3 biologically independent samples for each group. b, DNA on-target efficiency on site 1 of TadA–TadA*–Cas9n (wild-type TadA–evolved TadA heterodimer), TadAF148A–TadA*–Cas9n and TadAF148A–TadA*F148A–Cas9n. n = 3 biologically independent samples for each group. c, The number of RNA SNVs in the GFP, TadA–TadA*–Cas9n, TadAF148A–TadA*–Cas9n and TadAF148A–TadA*F148A–Cas9n groups. n = 3 biologically independent samples for each group. All values are presented as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001. Two-sided unpaired t-test. Exact P values are provided in Supplementary Table 17.
