Extended Data Fig. 2: Increased expression of deaminases induces an increase in off-target RNA SNVs.
From: Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis
a, Representative distributions of off-target RNA SNVs on human chromosomes for APOBEC1, BE3–site 3, BE3–RNF2, TadA–TadA*, ABE7.10–site 1 and ABE7.10–site 2. b, Schematics of BE3(FNLS) and ABEmax. Note that BE3(FNLS)31 and ABEmax32 have previously been reported to greatly increase the expression of base editors. c, Expression of APOBEC1 in cells transfected with BE3–site 3 for 36 or 72 h, or with BE3(FNLS)–site 3 for 72 h. d, Expression level of in cells transfected with ABE7.10–site 1 for 36 and 72 h, or with ABEmax–site 1 for 72 h. e, The number of off-target RNA SNVs in cells transfected with BE3–site 3 for 36 or 72 h, or with BE3(FNLS)–site 3 for 72 h. f, The number of off-target RNA SNVs in cells transfected with ABE7.10–site 1 for 36 or 72 h, or with ABEmax–site 1 for 72 h. Transfections: GFP for 36 h; BE3–site 3 for 36 h; GFP for 72 h; BE3–site 3 for 72 h; BE3(FNLS)–site 3 for 72 h; ABE7.10–site 1 for 36 h; ABE7.10–site 1 for 72 h; ABEmax–site 1 for 72 h. RSEM, RNA-seq by expectation maximization. All values are presented as mean ± s.e.m. Number above the bar indicates the number of biologically independent samples. *P < 0.05, **P < 0.01, ***P < 0.001, two-sided unpaired t-test. Exact P values are provided in Supplementary Table 15.
