Extended Data Fig. 4: Characteristics of off-target RNA SNVs.
From: Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis
a, Similarity between adjacent sequences of off-target RNA SNVs and on-target sequences. n = 2 biologically independent samples for BE3–site 2, n = 3 for BE3–RNF2, n = 3 for ABE7.10–site 1, n = 3 for ABE7.10–site 2. b, Sanger sequencing chromatograms show that C to U mutation was observed only in RNA but not DNA for two BE3 off-target sites. OF, off-target. Gene names, amino acid mutations and single nucleotide conversions are indicated by blue, red and green, respectively. c, Sanger sequencing chromatograms show that U to C mutation was observed only in the RNA of three ABE7.10 off-target sites.
