Extended Data Fig. 5: qPCR-based quantification of RNA-guided DNA integration efficiencies.
From: Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration
a, Potential lacZ transposition products in either orientation for both crRNA-3 and crRNA-4, and qPCR primer pairs to selectively amplify them. b, Comparison of simulated integration efficiencies for T-LR and T-RL orientations, generated by mixing clonally integrated and unintegrated lysates in known ratios, versus experimentally determined integration efficiencies measured by qPCR. c, Comparison of simulated mixtures of bidirectional integration efficiencies for crRNA-4, generated by mixing clonally integrated and unintegrated lysates in known ratios, versus experimentally determined integration efficiencies measured by qPCR. d, RNA-guided DNA integration efficiency as a function of IPTG concentration for crRNA-3 and crRNA-4, measured by qPCR. Data in b and c are shown as mean ± s.d. for n = 3 biologically independent samples.
