Extended Data Fig. 6: Influence of transposon end sequences on RNA-guided DNA integration.
From: Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration
a, Sequence (top) and schematic (bottom) of V. cholerae Tn6677 left- and right-end sequences. The putative TnsB-binding sites (blue) were determined based on sequence similarity to the TnsBbinding sites previously described14. The 8-bp terminal ends are shown in yellow, and the empirically determined minimum end sequences required for transposition are denoted by red dashed boxes. b, Integration efficiency with crRNA-4 as a function of transposon end length, as determined by qPCR. c, The relative fraction of both integration orientations as a function of transposon end length, determined by qPCR. ND, not determined. Data in b and c are shown as mean ± s.d. for n = 3 biologically independent samples.
