Extended Data Fig. 7: Analysis of RNA-guided DNA integration for PAM-tiled crRNAs and extended spacer length crRNAs.
From: Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration
a, Integration site distribution for all crRNAs described in Fig. 3d, e having a normalized transposition efficiency more than 20%, determined by NGS. b, Integration site distribution for a crRNA containing mismatches at positions 29–32, compared with the distribution with crRNA-4, determined by NGS. c, The crRNA-4 spacer length was shortened or lengthened by 6-nucleotide increments, and the resulting integration efficiencies were determined by qPCR. Data are normalized to crRNA-4 and are shown as mean ± s.d. for n = 3 biologically independent samples. d, Integration site distribution for extended length crRNAs compared with the distribution with crRNA-4, determined by NGS.
