Extended Data Fig. 4: Heart rate analysis.
a–c, Heart rate analysis before and after carbachol treatment (a) (*P < 0.05, two-sided paired t-test, n = 4 fish), mepyramine treatment (b) (*P < 0.05; two-sided paired t-test, n = 4 fish) and with or without sleep deprivation (c) (*P < 0.05; two-sided Student’s t-test, n = 7 non-sleep-deprived and 7 sleep-deprived fish). d–f, Histograms comparing the distribution of interbeat interval before (black bars) and after (red bars) treatment with carbachol (d) or mepyramine (e), and with (red bars) or without (black bars) sleep deprivation (f). Three representative histograms are shown for each condition. g, Representative images from heart rate capture showing ventricular contraction and the drawing of a mask (red) to measure fluorescence changes from the green fluorescent heartbeat. h, An illustrative plot of fluorescence variation measured by the mask, overlaid with the result of running peak analysis (red triangles) from which the heart rate and interbeat interval are derived.
