Extended Data Fig. 5: Brain-wide imaging with additional hypnotics.
a, Brain-wide single-plane imaging of promethazine-stimulated zebrafish. Promethazine induces bilaterally synchronous burst dynamics in the dorsal pallium (red bar). Left, masks used to extract Ca2+ transient traces shown in the middle panel. Dynamics are summarized by PCA (right). b, Brain-wide single-plane imaging of eserine-stimulated zebrafish. Eserine induces PMT waves. Left, masks used to extract Ca2+ transient traces shown in the middle panel. Dynamics are summarized by PCA (right). c, Brain-wide single-plane imaging of carbachol-stimulated zebrafish pre-incubated with methoctramine. Methoctramine prevents carbachol-induction of the PMT wave. Left, masks used to extract Ca2+ transient traces shown in the middle panel. Dynamics are summarized by PCA (right). d, Brain-wide single-plane imaging of eserine-stimulated zebrafish pre-incubated with methoctramine. Methoctramine prevents the eserine-induced PMT dynamics. Left, masks used to extract Ca2+ transient traces shown in the middle panel. Dynamics are summarized by PCA (right). e, Brain-wide single-plane imaging of zolpidem-stimulated zebrafish. Zolpidem induced multiple PMT waves. Left, masks used to extract Ca2+ transient traces shown in the middle panel. Dynamics are summarized by PCA (right). Red triangles show the point at which the drug was added. f, PCA by singular value decomposition of the fPSG PMT signature seen in Fig. 3e. Distinct steady states for wake (grey lines) and PWS sleep (black lines) can be separated, as can marked trajectories of muscle and brain travelling waves (blue lines). See Supplementary Information for reproducibility information. Scale bars, 100 μm.
