Extended Data Fig. 3: GoT captures genotyping information of single cells through cDNA.

a, Percentage of cells by number of UMIs with the CALR-mutation locus captured in standard 10x data (left panels) and GoT data (right panels) (see c for cell numbers in each sample). b, Number of UMIs per cell of CALR transcript from standard 10x data (blue shading) or targeted CALR locus from standard 10x or GoT (pink shading) (see c for cell numbers in each sample). c, Summary of clinical, pathological and GoT data from patients with CALR-mutated myeloproliferative neoplasms. BM, bone marrow; PB, peripheral blood. d, Number of genes per cell (left) and number of UMIs per cell (right) from published standard 10x data of healthy control CD34⁺ cells and 10x data from 3′ v.2 chemistry of CD34⁺ cells from patient samples that underwent concurrent GoT, after random downsampling of the reads from each sample to 50 million reads × 3 iterations, showing that the extra cycle of PCR and portioning a small aliquot from the 10x cDNA library for GoT using 3′ v.2 chemistry does not compromise scRNA-seq data.