Extended Data Fig. 4: Integration of samples from patients with essential thrombocythaemia and assignment of progenitor subsets.
From: Somatic mutations and cell identity linked by Genotyping of Transcriptomes
a, t-SNE projection of CD34+ progenitor cells from samples ET01–ET05, after integration and batch correction using the Seurat package (Methods). b, Heat map of top ten differentially expressed genes for clusters; lineage-specific genes from a previous publication26 are highlighted (Methods). c, Representative lineage-specific genes projected onto the t-SNE representation of CD34+ cells from samples from patients with essential thrombocythaemia. d, t-SNE projection of CD34+ cells from samples ET01–ET05 after applying a deep generative modelling approach for the single-cell analysis using the scVI package (Methods)19, showing assignments of progenitor subsets as determined after clustering the cells using the Seurat package. e, Genotyping data from GoT are projected onto the t-SNE representation generated after the scVI analysis of progenitor cells from samples ET01–ET05. Cells without any GoT data are labelled NA (not assignable).
