Extended Data Fig. 6: Intraperitoneal injection of LPS induces anti-OGDH CD8+ T cells in Pink1−/− mice.
From: Intestinal infection triggers Parkinson’s disease-like symptoms in Pink1−/− mice
Wild-type and Pink1−/− littermates were injected with 1 mg kg−1 of LPS intraperitoneally 4 times, once a week starting at 6 weeks of age. Spleens were obtained seven days after the last treatment. The presence of anti-OGDH CD8+ T cells was tested for via IFNγ ELISPOT by replacement of peptides in MHC class I complexes on splenocytes with OGDH peptides, control gB peptides or vehicle (water), and plated with splenic T cells on IFNγ detection plates. PMA peptide-loading controls to assess the levels of MHC class I expression in each group are shown in the inset. Data are representative of three independent experiments performed in triplicate. Significance was determined by two-way ANOVA at a 95% confidence interval with Tukey’s multiple comparison’s test. Data are mean ± s.e.m.
